1.Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.
2.Second, even if a microbe produces collagenase and other proteases, enzymes that break down proteins, they CAN'T reach the collagen protein due to its interaction with calcium.