1.Some are exchanged via small loops of DNA called plasmids.

因会通过被称为质粒的DNA小环交换。

2.Additionally to the genetic material contained within the nucleoid, many bacteria contain extra chromosomal DNA molecules called plasmids.

除了包含在拟中的遗传物质，许多细菌含有额的DNA分子，称为质粒。

3.Then they dropped the plasmid into a living bacterium, or yeast.

然后他们将质粒放入活细菌或酵母中。机翻

4.However, plasmids often contain genes that confer a selective advantage to the bacterium such as resistance to antibiotics.

不过质粒通常包含赋予细菌选择性优势的因，例如抗生素耐药性。

5.These plasmids are small double-stranded DNA molecules that can exist independently of the chromosome and they can be circular or linear.

它们是双链的DNA小分子，可以独立于细菌染色体（拟）而存在，可以是环状或线状的。

6.Transfer of the ligated plasmid into an E. coli strain (transformation).

7。转化成功的细菌的筛选。

7.The plasmid enters and multiplies itself within a cell and can then be transferred to others.

质粒进入细胞并在细胞内繁殖， 然后可以转移到其他细胞中。机翻

8.So essentially, these plasmids are the vehicles that transfer genes for antibiotic resistance between different bacterial species.

因此本质上，这质粒是在不同细菌物种之间转移抗生素抗性因的载体。机翻

9.This plasmid mutation has been seen in other places before; in China, in animals and some human patients.

这种质粒突变以前在其他地方也见过；在中国，在动物和人类患者中。机翻

10.The cDNAs had to be linked to prokaryotic transcription and translation signals and inserted into multicopy plasmids.

然而，占总蛋白30%的过量表达的蛋白质是沉淀或不溶的形式并且缺少翻译后修饰。

11.Plasmids have relatively few genes and their genetic information is not essential to the bacterium and cells that lack them usually function normally.

质粒所含的因相对较少，并且它们的因对细菌来说并不是必须的，缺少质粒的细胞往往也可以正常生长。

12.But this is the first time the plasmid has been found in the United States, and it could get passed around quickly.

但这是第次在美国发现这种质粒，它可能会很快传播开来。机翻

13.More specialized vectors have been engineered to use aspects of plasmids and bacteriophages, such as the plasmid-bacteriophage h hybrids known as cosmids

质粒和噬菌体已经在很多种类的细菌中被使用来表达因，并且噬菌体也许已经被用来将DNA整合到宿主因组上。

14.Phage h can be used to clone fragments larger than plasmid vectors, and phage M13 allows cloned DNA to be isolated in single-stranded form.

更多载体被改造能同时拥有质粒和噬菌体的特性，像被称为粘粒的质粒-噬菌体λ杂交体。

15.Or, in plain english, plasmids are part of the reason that, one day, antibiotics may not be able to help your strep throat anymore.

或者，用简单的英语来说，质粒是有天抗生素可能无法再帮助您治疗链球菌性咽喉炎的部分原因。机翻

16.I did all these experiments actually, with my own hands, I was 58 years old, I was still culturing plasmids and feeding cells, so it is very unlikely.

实际上，所有这实验都是我亲手做的，当时我 58 岁，我还在培养质粒和喂养细胞，所以这是非常不可能的。

17.The cytoplasm contains ribosomes which are the site of protein synthesis, the nucleoid which contains the genetic material, and plasmids which contain genes that confer a selective advantage to the bacterium.

细胞质中含有糖体，它们是蛋白质合成的部位；拟含有遗传物质以及质粒；质粒含有赋予细菌选择性优势的因。

18.But also, a much more terrifying explanation has to do with plasmids, which are parts of the bacterial DNA. The plasmid enters and multiplies itself within a cell and can then be transferred to others.

而且，个更可怕的解释与质粒有关，质粒是细菌 DNA 的部分。 质粒进入细胞并在细胞内自我繁殖， 然后可以转移到其他细胞中。机翻

19.And the thing that makes this mutation especially troubling is that it was found on a plasmid -- a little piece of DNA that can be easily shared between neighboring bacteria, meaning it can spread more easily.

让这种突变特别麻烦的是它是在质粒上发现的——小段 DNA 可以很容易地在相邻细菌之间共享，这意味着它可以更容易地传播。机翻