explant

1.It is the factors affected plant regeneration in explant culture in Euphorbia pulchrrima willd to different explants or aurin or cytokinin or the combination of the auxin and cytokinin.

外植体的类型、植物长素、细胞分裂素的类型及二者的不同组合均对在离体条件下植株的再具有影响。

2.To strip away periostea from calvaria by enzymes digesting separation and then culture explant in DMEM supplemented with 10% fetal calf serum.The new method is named floating explant culture.

在常规培养法的基础上进行改良，建立了组织块漂浮培养法，兔颅骨分离、培养成骨细胞。

3.Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.

法:先用IV型胶原酶和胰蛋白酶消化出3天的SD鼠臂层神经和坐骨神经组织块后再将组织块贴壁培养。