Methods To incubate the caries free permanent teeth with acid gelatinoid and/or collagenase, then to observe the ultrastructural change of root surface under scanning electron microscope .
方法将无龋恒牙分组经酸和酶分同孵化处理后切割制样,脱水干燥,喷金,扫描电镜观察根面超微结构的变化。
Methods To incubate the caries free permanent teeth with acid gelatinoid and/or collagenase, then to observe the ultrastructural change of root surface under scanning electron microscope .
方法将无龋恒牙分组经酸和酶分同孵化处理后切割制样,脱水干燥,喷金,扫描电镜观察根面超微结构的变化。
Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.
方法:先用IV型酶和胰蛋白酶消化出生3天的SD鼠臂层神经和坐骨神经组织块后再将组织块贴壁培养。
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