plasmid

1.pombe pREP series plasmids question...

主题： Re: [Pombelist] [Yeast] S.

2.Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

结果球孢链霉菌发酵液与溶菌均成功提取到质粒。

3.Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

方法利用球孢链霉菌发酵产生的变溶菌来消化双歧杆菌细胞壁，提取质粒，并与商业化溶菌法比较。

4.Cotransfection of GalT I promoter /luciferase reporter and Ets-1 expression plasmid increased the luciferase reporter activity in a dose dependent manner.

我们采用荧报告基因的方法证实Ets-1可激活 GalT I报告基因的表达，并具有量效关系。

5.3. 提取SOX5、SOX6和SOX9（SOX-5/6/9）基因的质粒，并包装至慢病毒（lentivirus）载体。

To package Sox 5, Sox 6 and Sox 9 plasmids （Sox-5/6/9） into lentivirus vectors. [Methods] 1.